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KMID : 0191120010160050579
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Journal of Korean Medical Science
2001 Volume.16 No. 5 p.579 ~ p.584
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Genotyping cagA, vacA subtype, iceA1, and babA of Helicobacter pylori isolates from Korean patients, and their association with gastroduodenal diseases
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Seok-Yong Kim
Chan Won Woo/Young-Min Lee/Bo Ra Son/Ji Won Kim/Hee Bok Chae/Sei Jin Youn/Seon Mee Park
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Abstract
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The genetic status of cagA, vacA subtype, iceA1, and babA, and the relationship to gastroduodenal
diseases were assessed in Helicobacter pylori isolates in Korea. Seventy-six strains of
H. pylori were isolated from the antrum and the corpus of 41 adult patients (22 with peptic
ulcer and 19 with gastritis). The cagA, iceA1, and babA genes were assessed by polymerase
chain reaction and the vacA subtypes were determined by reverse hybridization-line probe
assay. The positive rates of 349-bp cagA, 208-bp cagA, iceA1, and babA genes were 97.4%,
96.1%, 84.2%, and 36.1%, respectively. The vacA s1a, s1b, s1c, and s2 variants were detected
in 11.8%, 3.9%, 80.4%, and 1.3%, respectively. m1 (78.9%) is more prevalent than m2 (5.3%).
The most common vacA genotype was s1c/m1 (61.9%), and 14 isolates (18.4%) contained mixed
vacA genotypes from a single biopsy specimen. Twenty-one (60%) of 35 patients were infected
with more than two strains of different cagA, iceA1, babA, and vacA genotypes. None of cagA,
iceA1, babA, and vacA s1/m1 were associated with peptic ulcer. In conclusion, most H. pylori
isolates in Korea carry cagA, iceA1, and vacA s1c/m1 genes, and reside with multiple strains.
These genes do not correlate with the peptic ulcer in the Korean patients.
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